Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
Lucigens Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
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BL21-Gold Competent Cells 10 x 0.1mL For extremely toxic proteins use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based system. Efficiency is= 1 x 108 cfu/microgram of supercoiled DNA
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